Methods for the isolation of glycolytic intermediated by column chromatography with ion exchange resins.

In the course of the study of glycolysis in the human red blood cell which is reported in a companion paper (1) new information was collected on the application of column chromatography with anion exchange resins to the isolation of glycolytic intermediates and related compounds. The findings are the subject of this communication. Included in this report are modifications of some analytical methods for the determination of carbohydrate derivatives which proved helpful in the identification of the compounds found in column eluates. Columns of activated carbon were used to separate inorganic phosphate from the carbohydrate monophosphates in red cell extracts (I) and a brief discussion is presented on problems connected with carbon chromatography.